|
|
|
|
|
| Kadarusman,; Sugeha, Hagi Yulia; Pouyaud, Laurent; Hocdé, Régis; Hismayasari, Intanurfemi B.; Gunaisah, Endang; Widiarto, Santoso B.; Arafat, Gulam; Widyasari, Ferliana; Mouillot, David; Paradis, Emmanuel. |
| Coelacanth fishes of the genus Latimeria are the only surviving representatives of a basal lineage of vertebrates that originated more than 400 million years ago. Yet, much remains to be unveiled about the diversity and evolutionary history of these ‘living fossils’ using new molecular data, including the possibility of ‘cryptic’ species or unknown lineages. Here, we report the discovery of a new specimen in eastern Indonesia allegedly belonging to the species L. menadoensis. Although this specimen was found about 750 km from the known geographical distribution of the species, we found that the molecular divergence between this specimen and others of L. menadoensis was great: 1.8% compared to 0.04% among individuals of L. chalumnae, the other living... |
| Tipo: Text |
|
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00603/71466/69921.pdf |
| |
|
|
| Juhel, Jean-baptiste; Utama, Rizkie S.; Marques, Virginie; Vimono, Indra B.; Sugeha, Hagi Yulia; Kadarusman,; Pouyaud, Laurent; Dejean, Tony; Mouillot, David; Hocdé, Régis. |
| Environmental DNA (eDNA) has the potential to provide more comprehensive biodiversity assessments, particularly for vertebrates in species-rich regions. However, this method requires the completeness of a reference database (i.e. a list of DNA sequences attached to each species), which is not currently achieved for many taxa and ecosystems. As an alternative, a range of operational taxonomic units (OTUs) can be extracted from eDNA metabarcoding. However, the extent to which the diversity of OTUs provided by a limited eDNA sampling effort can predict regional species diversity is unknown. Here, by modelling OTU accumulation curves of eDNA seawater samples across the Coral Triangle, we obtained an asymptote reaching 1531 fish OTUs, while 1611 fish species... |
| Tipo: Text |
Palavras-chave: EDNA metabarcoding; Sequence clustering; Operational Taxonomic Unit; Diversity assessment; Detectability. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00640/75232/79601.pdf |
| |
|
|
| Delrieu‐trottin, Erwan; Durand, Jean‐dominique; Limmon, Gino; Sukmono, Tedjo; Kadarusman,; Sugeha, Hagi Yulia; Chen, Wei‐jen; Busson, Frédéric; Borsa, Philippe; Dahruddin, Hadi; Sauri, Sopian; Fitriana, Yuli; Zein, Mochamad Syamsul Arifin; Hocdé, Régis; Pouyaud, Laurent; Keith, Philippe; Wowor, Daisy; Steinke, Dirk; Hanner, Robert; Hubert, Nicolas. |
| DNA barcoding opens new perspectives on the way we document biodiversity. Initially proposed to circumvent the limits of morphological characters to assign unknown individuals to known species, DNA barcoding has been used in a wide array of studies where collecting species identity constitutes a crucial step. The assignment of unknowns to knowns assumes that species are already well identified and delineated, making the assignment performed reliable. Here, we used DNA‐based species delimitation and specimen assignment methods iteratively to tackle the inventory of the Indo‐Australian Archipelago grey mullets, a notorious case of taxonomic complexity that requires DNA‐based identification methods considering that traditional morphological identifications... |
| Tipo: Text |
Palavras-chave: Coral Triangle; Cryptic diversity; DNA barcoding; Reference library; Taxonomic gap. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71810/70300.pdf |
| |
|
|
| Cocquempot, Lucie; Delacourt, Christophe; Paillet, Jerome; Riou, Philippe; Aucan, Jérôme; Castelle, Bruno; Charria, Guillaume; Claudet, Joachim; Conan, Pascal; Coppola, Laurent; Hocdé, Régis; Planes, Serge; Raimbault, Patrick; Savoye, Nicolas; Testut, Laurent; Vuillemin, Renaud. |
| To understand and predict the physical, chemical, and biological processes at play in coastal and nearshore marine areas requires an integrated, interdisciplinary approach. The case study of the French structuration of coastal ocean and nearshore observing systems provides an original overview on a federative research infrastructure named ILICO. It is a notable example of national structuration and pan-institution efforts to investigate the forefront of knowledge on the processes at work within the critical coastal zone. ILICO comprises, in a pluridisciplinary approach, eight distributed network-systems of observation and data analysis that are accredited and financially supported by French research institutions and the French Ministry for Higher... |
| Tipo: Text |
Palavras-chave: Observation infrastructure; National structuration; Interdisciplinary; Coastal ocean; Coastline. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00503/61489/65292.pdf |
| |
|
|
| Polanco Fernández, Andrea; Marques, Virginie; Fopp, Fabian; Juhel, Jean‐baptiste; Borrero‐pérez, Giomar Helena; Cheutin, Marie‐charlotte; Dejean, Tony; González Corredor, Juan David; Acosta‐chaparro, Andrés; Hocdé, Régis; Eme, David; Maire, Eva; Spescha, Manuel; Valentini, Alice; Manel, Stéphanie; Mouillot, David; Albouy, Camille; Pellissier, Loïc. |
| Environmental DNA (eDNA) analysis is a revolutionary method to monitor marine biodiversity from animal DNA traces. Examining the capacity of eDNA to provide accurate biodiversity measures in species‐rich ecosystems such as coral reefs is a prerequisite for their application in long‐term monitoring. Here, we surveyed two Colombian tropical marine reefs, the island of Providencia and Gayraca Bay near Santa Marta, using eDNA and underwater visual census (UVC) methods. We collected a large quantity of surface water (30 L per filter) above the reefs and applied a metabarcoding protocol using three different primer sets targeting the 12S mitochondrial DNA, which are specific to the vertebrates Actinopterygii and Elasmobranchii. By assigning eDNA sequences to... |
| Tipo: Text |
Palavras-chave: Biodiversity; Biomonitoring; Caribbean Sea; Environmental DNA; Reef fishes; Underwater visual census. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00653/76543/77643.pdf |
| |
|
|
| Juhel, Jean‐baptiste; Marques, Virginie; Polanco Fernández, Andrea; Borrero‐pérez, Giomar H.; Mutis Martinezguerra, Maria; Valentini, Alice; Dejean, Tony; Manel, Stéphanie; Loiseau, Nicolas; Velez, Laure; Hocdé, Régis; Letessier, Tom B.; Richards, Eilísh; Hadjadj, Florine; Bessudo, Sandra; Ladino, Felipe; Albouy, Camille; Mouillot, David; Pellissier, Loïc. |
| Monitoring large marine mammals is challenging due to their low abundances in general, an ability to move over large distances and wide geographical range sizes. The distribution of the pygmy (Kogia breviceps) and dwarf (Kogia sima) sperm whales is informed by relatively rare sightings, which does not permit accurate estimates of their distribution ranges. Hence, their conservation status has long remained Data Deficient (DD) in the Red list of the International Union for Conservation of Nature (IUCN), which prevent appropriate conservation measures. Environmental DNA (eDNA) metabarcoding uses DNA traces left by organisms in their environments to detect the presence of targeted taxon, and is here proved to be useful to increase our knowledge on the... |
| Tipo: Text |
Palavras-chave: EDNA; Megafauna; Mobile species; Pelagic. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00683/79484/82076.pdf |
| |
|
|
|
